update model
continuous-integration/drone/push Build is passing Details

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- title: Engineering
icon: fa-solid fa-vial
details: In this study, we mainly constructed the target plasmid in vitro, amplified the required plasmid in Escherichia coli (Escherichia coli DH5α), and carried out the relevant transformation by the heat-shock transformation method; after obtaining the target plasmid, we then transformed it into the MG1655 strain, and then screened the target strains by colony PCR, enzyme digestion validation and so on.
details: In this study, we mainly constructed the target plasmid in vitro, amplified the required plasmid in Escherichia coli (Escherichia coli DH5α), and carried out the relevant transformation by the heat-shock transformation method.
link: ./engineering/README.md

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---
![](https://static.igem.wiki/teams/4657/wiki/assets/images/contribution.png)
Our project can provide a new idea based on synthetic biology for the treatment of acne, and make a contribution to the treatment and prevention of acne to a certain extent. We have worked hard to eliminate inequality and discrimination based on appearance. We expect that after the completion of our project in the future, our project can relieve people's physical discomfort caused by acne and acne, so that teenagers can grow up more healthy, happy, and relaxed, without fear of other people's strange eyes, and obtain mental health while maintaining physical health. As our original intention, we will surely overcome acne, overcome all inequalities, and leave the best memories of our youth.
Our project can provide a new idea based on synthetic biology for the treatment of acne, and make a contribution to the treatment and prevention of acne to a certain extent. We have worked hard to eliminate inequality and discrimination based on appearance. We expect that after the completion of our project in the future, our project can relieve people's physical discomfort caused by acne , so that teenagers can grow up more healthy, happy, and relaxed, without fear of other people's strange eyes, and obtain mental health while maintaining physical health. As our original intention, we will surely overcome acne, overcome all inequalities, and leave the best memories of our youth.
We make engineered bacteria recycling boxes by default, and recycle them after they play their role, in order to practice sustainable development, and reduce its pollution to the environment, so that vitamin B12 can play its role in a more suitable place, including disease treatment, etc., to maximize benefits, so that synthetic biology can enter the public vision, and more convenient to serve our lives.

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:::
We also analyzed on the problems caused by acne. According to our survey, acne has caused facial anxiety to 70.56% of people around us, 62.50% of people have facial discomfort. It also caused other problems such as social disorders and inferiority complex. Only 18.95% people think it doesn't cause any trouble.
We also analyzed the problems caused by acne. According to our survey, acne has caused facial anxiety to 70.56% of people around us, 62.50% of people have facial discomfort. It also caused other problems such as social disorders and inferiority complex. Only 18.95% people think it doesn't cause any trouble.
::: echarts The Problems Caused by Acne

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---
![](https://static.igem.wiki/teams/4657/wiki/assets/images/model.png)
$$
\text{Tetramer + Operator} \xrightleftharpoons[k_{l1'}]{k_{l1}} \text{TO}
\tag{L1}
$$
$$
\text{Lac + TO} \xrightleftharpoons[k_{l2'}]{k_{l2}} \text{LTO}
\tag{L2}
$$
$$
\text{LTO} \xrightarrow{k_{ccdB\text{-}m}} {mRNA_{ccdB}} \xrightarrow{(1-d_{ccdB\text{-}m})} {mRNA\varnothing} \xrightarrow{k_{ccdB}} {ccdB} \xrightarrow{(1-d_{ccdB})} {ccdB\varnothing}
\tag{L3}
$$

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## Lab Notes
<PDF url="https://static.igem.wiki/teams/4657/wiki/assets/notebook.pdf" />
## Wet Lab & Safety Notes
<PDF url="https://static.igem.wiki/teams/4657/wiki/assets/notebook2.pdf" />